A set of 13 polymorphic microsatellite markers were isolated and characterized from a genomic library enriched for dinucleotide repeats in the European pine marten Martes martes. Microsatellite loci amplification was tested on a panel of 12 tissue samples and 9 distinct hair samples collected from either road-killed or trapped animals in Tuscany, Italy. Allelic diversity was 6 and the number of alleles per locus ranged from 2 to 10. Mean observed and expected heterozygosity were 0.610 (range: 0.238-0.905) and 0.698 (range: 0.400-0.856), respectively. This novel set of microsatellite loci will be particularly useful for non-invasive genetic studies to assess population distribution and patterns of population structure and dispersal of M. martes in woodlands and fragmented habitats. © Springer Science+Business Media B.V. 2010.
Characterization of 13 polymorphic microsatellite loci in the European pine marten martes martes
Banchi E.;
2010-01-01
Abstract
A set of 13 polymorphic microsatellite markers were isolated and characterized from a genomic library enriched for dinucleotide repeats in the European pine marten Martes martes. Microsatellite loci amplification was tested on a panel of 12 tissue samples and 9 distinct hair samples collected from either road-killed or trapped animals in Tuscany, Italy. Allelic diversity was 6 and the number of alleles per locus ranged from 2 to 10. Mean observed and expected heterozygosity were 0.610 (range: 0.238-0.905) and 0.698 (range: 0.400-0.856), respectively. This novel set of microsatellite loci will be particularly useful for non-invasive genetic studies to assess population distribution and patterns of population structure and dispersal of M. martes in woodlands and fragmented habitats. © Springer Science+Business Media B.V. 2010.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
