Composition of Colloidal Organic Matter in Phytoplankton Exudates

The colloidal organic matter (COM) was isolated from the exudates of three cultured phytoplonkters, namely the chlophyte nanoflagellate Tetraselmis sp., the diatom Chaetoceros socialis and the dinoflagellate Prorocentrum minimum, from the Gulf of Trieste (northern Adriatic Sea). The isolation of COM was performed by ultrafiltration with molecular weight cut-off membranes of 5 kDa and final desalinisation by dialysis. The composition of the COM was characterised using C elemental analysis and H-1 NMR spectroscopy and compared with COM isolated from a marine sample from the same area (Gulf of Trieste). By using H-1 NMR spectroscopy, it was possible to semi-quantitatively determine the concentrations of the main biochemical constituents present in the COM samples. The results showed that the phytoplankton COM was predominantly composed of polysaccharides, with minor contributions from proteins and especially lipids. Therefore, the phytoplankton COM mainly contributes to the marine COM pool in the polysaccharide fraction and less in the protein and lipid fractions.