Delayed fluorescence as a measure of nutrient limitation in Dunaliella tertiolecta

The applicability of the delayed fluorescence (DF) for the purpose of distinguishing the cells growing in different nutrient conditions was researched on the marine unicellular algae Dunaliella tertiolecta Butcher (Chlorophyta). The DF intensity (DFI), as a measure of living algal biomass, was compared with other biomass measures – the cell concentration, chlorophyll a and fluorescence. The photosynthetic activity index (PhAI), a non-dimensional physiological index of photosynthesis calculated from a combination of DFI and F0 was introduced. The nitrogen deprivation was indicated by more than 50% drop of PhAI. DF decay kinetics was measured with two different illuminations (<600 nm and >650 nm). The measured curves were divided and the resulting peak utilized for the differentiation among nutrient conditions. DF decay kinetics of D. tertiolecta differed among the cells growing in various nutrient conditions, indicating changes in the photosynthesis physiology.